Genome maps of simian virus 40 defectives propagated in human glioblastoma cells

Abstract

The DNA sequences present in four defective SV40 genomes propagated on human glioblastoma cells (A172) have been characterized by analysis of restriction enzyme digests and by hybridization to the wild-type genome. Like defectives grown on monkey cells, these molecules are 10 to 20% shorter than wild-type DNA and contain reiterations of specific segments of the nondefective genome, including multiple copies of the viral replication origin. However, unlike the monkey cell defectives, those grown on A172 cells have also retained multiple copies of the region of viral DNA around the BamHl site at map position 0.15. These defectives consist only of sequences derived from SV40 and contain no detectable host cell sequences.