Genome-informed design of LAMP assays for specific detection of aster yellows phytoplasmas

Abstract

Genome sequence data is becoming more available and represents a source of potential target sequences for specific molecular tests to verify the feasibility and efficiency of genome-inform test design the attempt to identify novel specific sequences in the publicly available genomes of aster yellows phytoplasmas and use them to design primers for a spcific LAMP assays. Coupling RUCS automated analysis with PrimerExplorer V5 primer designer and applying criteria for selection of assays at each stage of the process resulted in 13 promising LAMP assays targeting four newly identified regions specific to aster yellows phytoplasmas.