Abstract
Currently, the molecular regulation mechanisms of disease-resistant involved in maize leaf sheaths infected by banded leaf and sheath blight (BLSB) are poorly known. To gain insight into the transcriptome dynamics that are associated with their disease-resistant, genome-wide gene expression profiling was conducted by Solexa sequencing. More than four million tags were generated from sheath tissues without any leaf or development leaf, including 193,222 and 204,824 clean tags in the two libraries, respectively. Of these, 82,864 (55.4 %) and 91,678 (51.5 %) tags were matched to the reference genes. The most differentially expressed tags with log2 ratio >2 or <−2 (P < 0.001) were further analyzed, representing 1,476 up-regulated and 1,754 down-regulated genes, except for unknown transcripts, which were classified into 11 functional categories. The most enriched categories were those of metabolism, signal transduction and cellular transport. Next, the expression patterns of 12 genes were assessed by quantitative real-time PCR, and it is showed the results were general agreement with the Solexa analysis, although the degree of change was lower in amplitude. In conclusion, we first reveal the complex changes in the transcriptome during the early development of maize sheath infected by BLSB and provide a comprehensive set of data that are essential for understanding its molecular regulation mechanism.Electronic supplementary materialThe online version of this article (doi:10.1007/s11033-014-3103-z) contains supplementary material, which is available to authorized users.
Highlights
Rhizoctonia solani Kuhn is a plant pathogenic fungus with a wide host range and worldwide distribution and has a significant economic impact in the development and production of a wide variety of crops
The variability in the degree of hyphal fusion, morphology, pathogenicity and host range observed in Anastomosis Groups (AGs) (AG-1, AG-13) and AG-1A has been widely endanger to maize as a dominant fungus in southwest of China and identified as anastomosis group [60]
To identify genes involved in maize sheaths infected by R. solani, two maize Solexa libraries were constructed from tissues of maize sheaths without any leaves or development leaves, including 4-d and 2-ck libraries
Summary
Rhizoctonia solani Kuhn is a plant pathogenic fungus with a wide host range and worldwide distribution and has a significant economic impact in the development and production of a wide variety of crops. Futhermore, R. solani Kuhn is a common soil borne pathogen with a great diversity of host plants that can attack previously resistant cultivars, along with the capacity of fungal spores to travel long distances, making control of BLSB difficult [61]. This disease initially infects maize at the first and second leaf sheath above the ground and spreads upward to infect the ear, leading to severe yield losses [61]. Yield losses approached 100 % in southern of China when the ear rot phase predominated [27]
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