Abstract
AbstractThe ever-increasing human population together with emerging environmental cues from climate change demand for novel innovations in plant breeding and agriculture. Targeted genome editing technologies especially the CRISPR/Cas systems have revolutionized basic research and crop breeding by enabling precise targeted modification of an organism’s genome. Lately, genome editing has been widely utilized in myriads of plant species to evaluate gene functions and improve valued agronomic traits including pathogen resistance, abiotic tolerance, yield and quality. In this chapter, we provide a brief overview of genome editing technologies with a special focus on CRISPR/Cas systems. In addition, we discuss about novel innovations in CRISPR-based technologies and their subsequent usage in the development and commercialization of improved genome-edited crops. Further, we have also pointed out major challenges faced by plant genome editing and have predicted possible solutions for speeding of plant breeding and improving crop productivity.KeywordsGenome editingCRISPR/CasTargeted mutagenesisPlant breedingCrop improvementBase editingPrime editingAgronomic traits
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