Abstract
Jatropha dioica is traditionally used owing to its antiviral, antifungal, and antimicrobial properties. But, toxicological information regarding J. dioica root total extract is currently limited. The aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract. Three different J. dioica aqueous root total extract doses (60, 100, and 300 mg/kg) were administered orally to Wistar rats during 5 days through the pregnancy term (16–21 days). Pregnant rats were sampled every 24 h during the last 6 days of gestation, and pubs were sampled at birth. Genome damage in dams and their newborn pups transplacentally exposed to J. dioica was evaluated by in vivo micronuclei assay. We evaluated the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE), and polychromatic erythrocytes (PCE) in peripheral blood samples from pups and MNPCE and PCE in pregnant rats. No genotoxic effect was observed after oral administration of the three different doses of aqueous root total extract of J. dioica in pregnant or in their newborn pubs, after transplacental exposure. A significant decrease in PCE frequency was noted in samples from pubs of rats treated with the highest dose of J. dioica extract. The aqueous total root extract of J. dioica at the highest dose tested in our research do have cytotoxic effect in pups transplacentally exposed to this plant extract. Moreover, neither a genotoxic nor a cytotoxic effect was observed in pregnant rats. In the present work, there was no evidence of genome damage in the rat model after transplacental exposure to J. dioica aqueous root total extract.
Highlights
IntroductionE aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract
Gabriela Morales-Velazquez,1,2 Blanca Patricia Lazalde-Ramos,3 Belinda Claudia Gomez-Meda,4 Guillermo Moises Zuñiga-Gonzalez,5 Yveth Marlene Ortiz-Garcıa,1 Rosalinda Gutierrez-Hernandez,6 Celia Guerrero-Velazquez,1 Susana Vanessa Sanchez de la Rosa,1 and Ana Lourdes Zamora-Perez 1
We evaluated the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE), and polychromatic erythrocytes (PCE) in peripheral blood samples from pups and MNPCE and PCE in pregnant rats
Summary
E aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract. No genotoxic effect was observed after oral administration of the three different doses of aqueous root total extract of J. dioica in pregnant or in their newborn pubs, after transplacental exposure. E aqueous total root extract of J. dioica at the highest dose tested in our research do have cytotoxic effect in pups transplacentally exposed to this plant extract. There was no evidence of genome damage in the rat model after transplacental exposure to J. dioica aqueous root total extract. Is plant presents many positive health effects including antiviral, antifungal, and antimicrobial activities, and the root of the plant is chewed to treat buccal diseases, for example, in tooth mobility [5]. Regardless of the frequent use of natural products, there are lack of studies that provide information about the safe use of medicinal plants, since plants could present a therapeutic potential, and adverse effects [13]
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