Genome-Based Classification of Strain 16-SW-7, a Marine Bacterium Capable of Converting B Red Blood Cells, as Pseudoalteromonas distincta and Proposal to Reclassify Pseudoalteromonas paragorgicola as a Later Heterotypic Synonym of Pseudoalteromonas distincta.

Olga I. Nedashkovkaya,Liudmila A. Tekutyeva,Oksana M. Son,Natalia V. Zhukova,Valery V. Mikhailov,Song-Gun Kim,Larissa A. Balabanova

doi:10.3389/fmicb.2021.809431

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped, and motile bacterium, designated strain 16-SW-7, isolated from a seawater sample, was investigated in detail due to its ability to produce a unique α-galactosidase converting B red blood cells into the universal type blood cells. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain 16-SW-7 is a member of the Gammaproteobacteria genus Pseudoalteromonas. The closest relatives of the environmental isolate were Pseudoalteromonas distincta KMM 638T and Pseudoalteromonas paragorgicola KMM 3548T, with the plural paralogous 16S rRNA genes of 99.87–100% similarity. The strain 16-SW-7 grew with 1–10% NaCl and at 4–34°C, and hydrolyzed casein, gelatin, tyrosine, and DNA. The genomic DNA G+C content was 39.3 mol%. The prevalent fatty acids were C16:1 ω7c, C16:0, C17:1 ω8c, C18:1 ω7c, C17:0, and C12:0 3-OH. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, two unidentified amino lipids, and three unidentified lipids. The major respiratory quinone was Q-8. The finished genome of the strain 16-SW-7 (GenBank assembly accession number: GCA_005877035.1) has a size of 4,531,445 bp and comprises two circular chromosomes L1 and S1, deposited in the GenBank under the accession numbers CP040558 and CP040559, respectively. The strain 16-SW-7 has the ANI values of 98.2% with KMM 638T and KMM 3548T and the DDH values of 84.4 and 83.5%, respectively, indicating clearly that the three strains belonged to a single species. According to phylogenetic evidence and similarity for the chemotaxonomic and genotypic properties, the strain 16-SW-7 (= KCTC 52772 = KMM 701) represents a novel member of the species Pseudoalteromonas distincta. Also, we have proposed to reclassify Pseudoalteromonas paragorgicola as a later heterotypic synonym of P. distincta based on the rules of priority with the emendation of the species.

Highlights

The genus Pseudoalteromonas was proposed by Gauthier et al (1995) by splitting the genus Alteromonas into two genera due to its high level of heterogeneity
We clarify the taxonomic position of the strain 16-SW-7, reclassify the species P. paragorgicola as a later heterotypic synonym of P. distincta, and specify the description of the species P. distincta based on the results of phylogenetic analysis, and genotypic and phenotypic characterization
The type strains P. distincta KMM 638T (=ATCC 700518T) and P. paragorgicola KMM 3548T (=DSM 26439T) were obtained from the collection of marine microorganisms (KMM) and used as the reference strains for comparative taxonomic analysis

Summary

INTRODUCTION

The genus Pseudoalteromonas was proposed by Gauthier et al (1995) by splitting the genus Alteromonas into two genera due to its high level of heterogeneity. Members of the genus Pseudoalteromonas are often isolated from different marine environments, including surface and deep seawater, sediments and sea ice samples, ascidians, coral, a surface slime of a puffer fish, mussels, brown and green algae, diatoms, and the halophyte plants (Bowman, 1998; Sawabe et al, 2000; Egan et al, 2001; Ivanova et al, 2002b,c, 2004; Romanenko et al, 2003a,b; Park et al, 2005; Matsuyama et al, 2013; Wu et al, 2017; Navarro-Torre et al, 2020). The type and single strain of P. distincta KMM 638T (formerly Alteromonas distincta) was originally isolated from a marine sponge collected at a depth of 350 m near the Komandorskie Islands, Russia (Romanenko et al, 1995). This strain formed non-pigmented colonies and was shown to produce the dark-gray diffusible melanin-like pigments. We clarify the taxonomic position of the strain 16-SW-7, reclassify the species P. paragorgicola as a later heterotypic synonym of P. distincta, and specify the description of the species P. distincta based on the results of phylogenetic analysis, and genotypic and phenotypic characterization

MATERIALS AND METHODS

RESULTS AND DISCUSSION

DATA AVAILABILITY STATEMENT