Abstract
Infectious hematopoietic necrosis virus (IHNV), one of the most important pathogenic fish viruses, affects trout fisheries and causes considerable economic losses. Currently, in Korea, more studies on IHNV infection are being reported. However, relatively less data is available on Korean isolates than on those from other countries. Few studies have focused on gene sequence analyses of IHNV glycoprotein (G) gene and almost none have focused on other gene fragments. Therefore, considering the dearth of adequate phylogenetic and genomic studies on Korean IHNV strains because of the lack of data, our study aimed to provide sufficient relevant data by sequencing the complete genome of the IHNV strain SNU1, which was recently isolated from a Korean rainbow trout farm. Moreover, we focused on expanding the perspectives on the phylogenesis of IHNV isolates from Korea and other Asian countries. IHNV was isolated from pooled hematopoietic tissue samples using Epithelioma papulosum cyprinid (EPC) cells, and phylogenetic analysis and genome study were conducted using complete G, N, and nonvirion (NV) gene sequences. Our main achievements were the development of a phylogenetic analytical method based on the NV gene and complete genome sequence analysis of the IHNV strain SNU1, which was compared with other Asian isolate sequences.
Highlights
Infectious hematopoietic necrosis virus (IHNV), one of the most devastating fish pathogens, causes considerable economic losses to the aquaculture industry [1]
Studies focused on the genome analysis of IHNV isolates have been conducted using various methods in other countries, but not for Korean isolates
Since the occurrence rate of IHNV infection has increased in the 21st century in Korea, the need for a study focused on the phylogenesis and evolution of Korean isolates is urgent to develop a vaccine that can be used to protect rainbow trout fish in Korea
Summary
Infectious hematopoietic necrosis virus (IHNV), one of the most devastating fish pathogens, causes considerable economic losses to the aquaculture industry [1]. The virus was first reported in the 1950s in sockeye salmon (Oncorhynchus nerka) from Washington and Oregon fish hatcheries in the United States [3]. Diverse fish species are known to be susceptible to IHNV infections including salmonids, which are the most vulnerable [4]. The pathogenicity of the virus is known to depend on environmental conditions and the size and species of the fish [1]. The virus was first reported in Asian countries in 1971 when diseased kokanee salmon (freshwater O. nerka) were imported to Japan from North America [6]
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