Genome and Bioinformatic Analysis of a HAdV-B14p1 Virus Isolated from a Baby with Pneumonia in Beijing, China

Liuying Tang,Yixin Ji,Shoaleh Dehghan,Junjing An,Wenbo Xu,Zhengde Xie,Donald Seto,Amit Kapoor

doi:10.1371/journal.pone.0060345

Abstract

The genome of HAdV-B14p1 strain BJ430, isolated from a six-month-old baby diagnosed with bronchial pneumonia at the Beijing Children’s Hospital in December 2010, was sequenced, analyzed, and compared with reference adenovirus genome sequences archived in GenBank. This genome is 34,762 bp in length, remarkably presenting 99.9% identity with the genome from HAdV14p1 strain 303600, which was isolated in the USA (2006). Even more remarkable, it is 99.7% identical with the HAdV-B14p (prototype “de Wit” strain) genome, isolated from The Netherlands in 1955. The patient and its parents presumably had no or limited contact with persons from the USA and Ireland, both of which reported outbreaks of the re-emergent virus HAdV-14p1 recently. These genome data, its analysis, and this report provide a reference for any additional HAdV-B14 outbreak in China and provide the basis for the development of adenovirus vaccines and molecular pathogen surveillance protocols in high-risk areas.

Highlights

Human adenoviruses(HAdVs)are typed and ordered into seven species (A to G) with greater than 64 genome types reported in which the associations for specific human diseases are characterized [1,2]
HAdV-B14p was first isolated from The Netherlands and linked to a respiratory tract disease outbreak in military recruits between April and May, 1955[4]. This particular virus was associated with sporadic cases of acute respiratory disease (ARD) in Europe and Asia through the 1960s [4,5] and was not reported for a long period
Specific primers were designed for sequencing the open reading frames of the E1A, hexon, and fiber genes. Virus from this and other clinical samples were grown in Hep-2 cells; this sample presented a characteristic cytopathic effect (CPE), which was observed after 10 days of culturing

Summary

Introduction

Human adenoviruses(HAdVs)are typed and ordered into seven species (A to G) with greater than 64 genome types reported in which the associations for specific human diseases are characterized [1,2]. Given the recent outbreaks of this particular virus in the USA, Europe and China, these genome data and this report provide a reference for recognizing any future HAdV-B14 outbreak in China and serves as a foundation for the development of adenovirus vaccines and surveillance protocols in high-risk areas. These observations will aid the continuing research and development of adenovirus-based vectors

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Results

Conclusion