Abstract
Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens. Nicotinamide adenine dinucleotide (NAD)-independent MS was first reported in 1975. Despite the atypical traits of NAD-independent MS, its independence from NAD has not been studied. In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. The assembled genomes were compared with the genomes of MS-H vaccine strain and type strain WVU1853. We found that the coding sequences of nicotinate phosphoribosyltransferase and glycerol-3-phosphate acyltransferase, and a unique coding sequence were present only in the genomes of NAD-independent isolates.
Highlights
Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens [1]
Five MS strains capable of growing in Modified Frey’s Broth (MFB) without nicotinamide adenine dinucleotide (NAD) were isolated. To confirm their NAD-independence, the MS isolates, type strain WVU1853 and vaccine strain MS-H were incubated in MFB without NAD supplement and with NAD (Sigma-aldrich, Saint Louis, MO, USA) supplement, respectively
The growth of MS isolates obtained from Korea in NAD-negative broth was measured (Table 1)
Summary
Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens [1]. Ever since it was first reported in 1954 by Olson et al [2], many studies have been conducted to isolate and incubate MS. MS strains that do not require NAD for growth have been reported several times. To understand the NAD independence of the MS isolates, we performed comparative genome analyses on the isolates using their newly determined complete genomes. Comparative analyses were performed to discover the unique coding sequences (CDSs) present on NAD-independent MS strains
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