Abstract
A nucleotide stretch located upstream of the MRAS3 gene of the zygomycete Mucor racemosus was found to hybridize to multiple restriction fragments of genomic DNA of the ascomycete Podospora anserina. DNA of strains derived from different geographical locations revealed no major restriction length fragment polymorphisms. Also, no age-related polymorphisms were observed. The repetitive sequence is located on all of the physically fractionated Podospora chromosomes. Sequence analysis of the upstream sequence of MRAS3 and of a DNA-stretch from a genomic clone of P. anserina which hybridized to the Mucor probe revealed a region of sequence homology. This sequence is composed of alternating G and T residues [poly(dG-dT) or poly(GT)] of 76 bp in length and thus represents a simple repetitive sequence, or microsatellite. A synthetic oligonucleotide, (GT) 8, corresponding to this sequence gave also rise to a complex hybridization pattern. Screening of genomic libraries of P. anserina revealed poly(GT)-stretches on about 5–7% of all clones. Finally, this repetitive sequence was identified in the genome of the filamentous fungi Sordaria macrospora and Curvularia lunata.
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