Abstract
Excretory/Secretory (ES) proteins play an important role in the host-parasite interactions. Experimental identification of ES proteins is time-consuming and expensive. Alternative bioinformatics approaches are cost-effective and can be used to prioritize the experimental analysis of therapeutic targets for parasitic diseases. Here we predicted and functionally annotated the ES proteins in T. solium genome using an integration of bioinformatics tools. Additionally, we developed a novel measurement to evaluate the potential antigenicity of T. solium secretome using sequence length and number of antigenic regions of ES proteins. This measurement was formalized as the Abundance of Antigenic Regions (AAR) value. AAR value for secretome showed a similar value to that obtained for a set of experimentally determined antigenic proteins and was different to the calculated value for the non-ES proteins of T. solium genome. Furthermore, we calculated the AAR values for known helminth secretomes and they were similar to that obtained for T. solium. The results reveal the utility of AAR value as a novel genomic measurement to evaluate the potential antigenicity of secretomes. This comprehensive analysis of T. solium secretome provides functional information for future experimental studies, including the identification of novel ES proteins of therapeutic, diagnosis and immunological interest.
Highlights
Excretory/Secretory (ES) proteins play an important role in the host-parasite interactions
Of the 12,902 proteins encoded in the T. solium genome[26], we could annotate a total of 731 proteins as classical secretory proteins by SignalP35 and 543 proteins as non-classical secretory proteins by SecretomeP36
The ES proteins were searched against the RNAseq and ESTs libraries from T. solium to analyze the percentage of ES proteins that are supported at RNA level
Summary
Excretory/Secretory (ES) proteins play an important role in the host-parasite interactions. We developed a novel measurement to evaluate the potential antigenicity of T. solium secretome using sequence length and number of antigenic regions of ES proteins This measurement was formalized as the Abundance of Antigenic Regions (AAR) value. The results reveal the utility of AAR value as a novel genomic measurement to evaluate the potential antigenicity of secretomes This comprehensive analysis of T. solium secretome provides functional information for future experimental studies, including the identification of novel ES proteins of therapeutic, diagnosis and immunological interest. We developed a novel genomic measurement to evaluate the potential antigenicity of a secretome using the sequence length and the number of antigenic regions of ES proteins This measurement was formalized as the Abundance of Antigenic Region (AAR) value. Our work represents a starting point to the characterization of the parasite secretome and it would contribute to a better comprehension of the host-parasite interactions
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