Abstract
In the present work, we analyse the genomic and phenotypic characteristics of a strain named RZME27T isolated from roots of a Zea mays plant grown in Spain. The phylogenetic analyses of 16S rRNA gene and whole genome sequences showed that the strain RZME27T clustered with the type strains of Neorhizobium galegae and Pseudorhizobium pelagicum from the family Rhizobiaceae. This family encompasses several genera establishing symbiosis with legumes, but the genes involved in nodulation and nitrogen fixation are absent in its genome. Nevertheless, genes related to plant colonization, such as those involved in motility, chemotaxis, quorum sensing, exopolysaccharide biosynthesis and hydrolytic enzymes production were found. The comparative pangenomic analyses showed that 78 protein clusters present in the strain RZME27T were not found in the type strains of its closest relatives N. galegae and P. pelagicum. The calculated average nucleotide identity (ANI) values between the strain RZME27T and the type strains of N. galegae and P. pelagicum were 75.61% and 75.1%, respectively, similar or lower than those found for other genera from family Rhizobiaceae. Several phenotypic differences were also found, highlighting the absence of the fatty acid C19:0 cyclo ω8c and propionate assimilation. These results support the definition of a novel genus and species named Endobacterium cerealis gen. nov. sp. nov. whose type strain is RZME27T.
Highlights
The family Rhizobiaceae [1] belongs to the class Alphaproteobacteria and currently, this family comprises the classical genera Rhizobium [2], Agrobacterium [3], Sinorhizobium [4], and Allorhizobium [6] as well as the more recently described genera Shinella [7], Ciceribacter [8], Neorhizobium [9], Pararhizobium [10], Pseudorhizobium [11], Gellertiella [12] and Georhizobium [13]
The comparison against GenBank of the 16S rRNA gene showed that the strain RZME27T belongs to the family Rhizobiaceae and that it is equidistant to different species of genera Neorhizobium and Microorganisms 2020, 8, x FOR PEER REVIEW
We reported the genomic and phenotypic characteristics of a strain named RZME27T isolated from Zea mays roots growing in a field located at León (Northern Spain)
Summary
The family Rhizobiaceae [1] belongs to the class Alphaproteobacteria and currently, this family comprises the classical genera Rhizobium [2], Agrobacterium [3], Sinorhizobium [4] (later transferred to Ensifer [5]), and Allorhizobium [6] as well as the more recently described genera Shinella [7], Ciceribacter [8], Neorhizobium [9], Pararhizobium [10], Pseudorhizobium [11], Gellertiella [12] and Georhizobium [13] Several of these genera contain species originally isolated from legume nodules, such as Rhizobium, Ensifer (Sinorhizobium), Allorhizobium, Shinella, Neorhizobium or Pararhizobium [14,15,16] and from plant tumours such as Agrobacterium [17]. The aims of this study were the genome analysis of this novel genus for which we proposed the name Endobacterium gen. nov. and the characterization through the analysis of genomic, chemotaxonomic and phenotypic traits of its first described species Endobacterium cerealis gen. nov. sp. nov., whose type strain, RZME27T, has been deposited in two international culture collections under the accession numbers LMG 31256T and CECT 9794T
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