Abstract
A halophilic marine bacterial strain, PS-C1, was isolated from Sekinchan beach in Selangor, Malaysia. The 16S rRNA gene sequence analysis indicated that strain PS-C1 was associated with the genus Celeribacter. To date, there have been no reports on enzymes from the genus Celeribacter. The present study reports on the cellular features of Celeribacter sp. PS-C1, its annotated genome sequence, and comparative genome analyses of Celeribacter glycoside hydrolase (GH) enzymes. The genome of strain PS-C1 has a size of 3.87 Mbp and a G+C content of 59.10%, and contains 3739 protein-coding genes. Detailed analysis using the Carbohydrate-Active enZYmes (CAZy) database revealed that Celeribacter genomes harboured at least 12 putative genes encoding industrially important GHs that are grouped as cellulases, β-glucanases, hemicellulases, and starch-degrading enzymes. Herein, the potential applications of these enzymes are discussed. Furthermore, the activities of two types of GHs (β-glucosidase and licheninase) in strain PS-C1 were demonstrated. These findings suggest that strain PS-C1 could be a reservoir of novel GH enzymes for lignocellulosic biomass degradation.
Highlights
Marine environments are home to complex and diverse microorganisms that are yet to be discovered through appropriate microbial investigations
The 16S rRNA gene sequence (1453 bp) of strain PS-C1 was searched against the NCBI GenBank and EzBioCloud
The results showed that strain PS-C1 was a halophile, similar to all members of the genus Celeribacter (Table 1)
Summary
Marine environments are home to complex and diverse microorganisms that are yet to be discovered through appropriate microbial investigations. Marinederived halophilic bacteria are being explored to harness their novel enzymes and bioactive compounds as substitutes for many industrial applications [1,2,3,4]. The Rhodobacteraceae family is one of the bacterial lineages in marine ecosystems [5], and currently consists of 191 genera that are archived in the List of Prokaryotic names with Standing in Nomenclature (LPSN) database Unlike the major industrial microbes of the Bacillaceae family [6,7,8], reports on the potential applications of members of the family Rhodobacteraceae are relatively scarce [9]. Celeribacter is one of the least studied genera in Rhodobacteraceae, with no reports available on its industrial-related enzymes.
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