Abstract
Matsumuraeses phaseoli is a Lepidopteran pest that primarily feeds on numerous species of cultivated legumes, such as Glycine and Phaseolus. It is widely distributed in northeast Asia. A novel granulovirus, designated as Matsumuraeses phaseoli granulovirus (MaphGV), was isolated from pathogenic M. phaseoli larvae that dwell in rolled leaves of Astragalus membranaceus, a Chinese medicinal herb. In this study, using next-generation sequencing, we report the complete genome of MaphGV. MaphGV genome comprises a double-stranded DNA of 116,875 bp, with 37.18% GC content. It has 128 hypothetical open reading frames (ORFs). Among them, 38 are baculovirus core genes, 18 are lepidopteran baculovirus conserved genes, and 5 are unique to Baculoviridae. MaphGV has one baculovirus repeat ORF (bro) and three inhibitors of apoptosis proteins (iap), including a newfound iap-6. We found two atypical baculoviral homologous regions (hrs) and four direct repeats (drs) in the MaphGV genome. Based on phylogenetic analysis, MaphGV belongs to Clade b of Betabaculovirus and is closely related to Cydia pomonella granulovirus (CpGV) and Cryptophlebia leucotreta granulovirus (CrleGV). This novel baculovirus discovery and sequencing are invaluable in understanding the evolution of baculovirus and MaphGV may be a potential biocontrol agent against the bean ravaging pest.
Highlights
Baculoviruses are double-strand DNA viruses that infect the larvae of insect orders such as Lepidoptera, Hymenoptera, and Diptera [1]
The target mitochondrial COI barcode was amplified by polymerase chain reaction (PCR) using lepidopteran COI primers (Table S1) [13]
Baculoviruses were named according to the host species from which they were first isolated, followed by their occlusion bodies (OBs) morphology group [44]
Summary
Baculoviruses are double-strand DNA viruses that infect the larvae of insect orders such as Lepidoptera, Hymenoptera, and Diptera [1]. The family Baculoviridae is divided into four genera. There is much less genomic information for Beta-, Gamma-, and Deltabaculovirus than for Alphabaculovirus. The occlusion bodies (OBs) of Betabaculovirus are ovocylindrical structures, generally 0.12 × 0.50 μm in diameter, and are significantly smaller than those of Alphabaculovirus (0.15 to 5 μm) [2]. The genus was initially called granuloviruses (GVs) due to the granule-like morphology of the OBs [3]. Each virion of occlusion-derived virus (ODV) typically contains a single nucleocapsid within a single envelope
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
