Genome Analysis of a Natural Hybrid with 2n= 63 Chromosomes in the Genus Elytrigia Desv. (Poaceae) Using the GISH Technique

Abstract

Abstract: Genomic in situ hybridization (GISH), using genomic DNA probes from Thinopyrum elongatum (E genome, 2n= 14), Th. bessarabicum (J genome, 2n= 14), Pseudoroegneria stipifolia (S genome, 2n= 14), Agropyron cristatum (P genome, 2n= 28) and Critesion californicum (H genome, 2n= 14), was used to identify the genome constitution of a natural hybrid population morphologically close to Elytrigia pycnantha and with somatic chromosome number of 2n= 63. The GISH results indicated the presence of a chromosomal set more or less closely related to the E, P, S and H genomes. In particular, two sets of 14 chromosomes each showed close affinity to the E genome of Th. elongatum and to the P genome of A. cristatum. However, they included 2 and 10 mosaic chromosomes, respectively, with S genome specific sequences at their centromeric regions. Two additional sets (28 chromosomes) appeared to be very closely related to the S genome of Ps. stipifolia. The last genome involved (7 chromosomes) is related to the H genome of C. californicum but includes one chromosome with S genome‐specific sequences around the centromere and two other chromosomes with a short interstitial segment also containing S genome related sequences. On a basis of GISH analysis and literature data, it is hypothesized that the natural 9‐ploid hybrid belongs to the genus Elytrigia and results from fertilization of an unreduced gamete (n = 42) of E. pycnantha and a reduced gamete (n = 21) of E. repens. The genomic formula SSSSPSPSESESHS is proposed to describe its particular genomic and chromosomal composition.