Abstract
The genome sequence of Thermococcus gammatolerans, a radioresistant archaeon, is described; a proteomic analysis reveals that radioresistance may be due to unknown DNA repair enzymes.
Highlights
Thermococcus gammatolerans was isolated from samples collected from hydrothermal chimneys
Genome annotation accuracy as evaluated by proteomics We analyzed the proteome content of T. gammatolerans grown in optimal conditions at two stages, exponential and stationary
Our experimental results clearly show that all MS/MS identified peptides map to an entry in both the TGAM_ORF0 and TGAM_CDS1 databases, corresponding to 44% of the theoretical proteome and to a polypeptide coverage of 33% on average
Summary
Thermococcus gammatolerans was isolated from samples collected from hydrothermal chimneys. Thermococcales are strictly anaerobic and hyperthermophilic archaea belonging to the Euryarchaeota phylum In this order, three genera are distinguished: Pyrococcus [1], Thermococcus [2] and Palaeococcus [3]. With about 180 different species listed to date, the Thermococcus genus is the largest archaeal group characterized so far They have been isolated from terrestrial hot springs, deep oil reservoirs, and are widely distributed in deep-sea environments [4,5]; they are considered as key players in marine hot-water ecosystems. Several species are capable of fermenting peptides, amino acids or carbohydrates without sulfur producing acids, CO2 and H2 as end products [6,7] Some species such as Thermococcus strain AM4 and Thermococcus onnurineus NA1 were shown to be capable of lithotrophic growth on carbon monoxide [8,9]. The CO molecule, probably oxidized into CO2, is used as energy and/ or carbon source
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