Genodiagnosis of HPV beta DNA association with seborrheic keratosis in immunosuppressed and immunocompetent patients

Abstract

Numerous data indicate the involvement of human papilloma virus (HPV), genus beta, in the development of epithelial tumors of the skin. We studied the association of seborrheic keratosis (SK) with HPV by analysis of HPV beta DNA, including that in immunosuppressed patients. Twenty recipients of renal transplants and 20 immunocompetent patients with SK were examined. Control group consisted of 49 volunteers without epithelial tumors of the skin. Genodiagnosis of HPV beta DNA in biopsy specimens was carried out by the real time polymerase chain reaction with hybridization fluorescent detection. Amplification and detection were carried out on a Rotor-Gene 3000 device (Corbett Research, Australia). The results indicated high levels of HPV DNA in transplant recipients - in foci of SK and in visually intact skin (in 85 and 55% cases, respectively), which was higher than the incidence of the virus in donor skin (47%). Virus charge in SKfoci was significantly higher than in the control group.