Abstract
Genistein (4′, 5, 7-trihydroxyisoflavone) is an isoflavone compound obtained from plants that has potential applications in cancer therapy. However, the molecular mechanism of the action of genistein on cancer cell apoptosis is not well known. In this study, we investigated the effect of genistein on topoisomerase II-α (Topo IIα), an important protein involved in the processes of DNA replication and cell proliferation. The results revealed that inhibition of Topo IIα expression through the regulation of Specificity protein 1 and Specificity protein 3 may be one of the reasons for genistein’s induction of HeLa cell apoptosis.
Highlights
Genistein is an isoflavone compound obtained from plants which has many physiological functions such as estrogen action, anti-oxidation, mutation prevention, anti-infection, prevention and therapy of heart-cerebrovascular disorders, etc. [1,2,3], which suggest its usage in health care.Int
methylthiazolyl tetrazolium (MTT) Assay for Genistein Inhibition of HeLa Cell
To investigate the effect of genistein on Topo IIα expression at gene transcription level, HeLa cells were treated with genistein for 48 h and the expression of Topo IIα, Sp1 and Sp3 mRNA in HeLa cells were examined with RT-PCR
Summary
Genistein is an isoflavone compound obtained from plants which has many physiological functions such as estrogen action, anti-oxidation, mutation prevention, anti-infection, prevention and therapy of heart-cerebrovascular disorders, etc. [1,2,3], which suggest its usage in health care.Int. Topoisomerase II (Topo II) is a ubiquitous nuclear protein catalyzing the reaction of breakage and relinking of DNA, which plays an important role in maintenance of DNA topology required for DNA replication, transcription, and recombination of cellular genes. The two Topo II isoforms, Topo IIα and Topo IIβ, are 170 kDa and 180 kDa proteins, respectively, and have different roles in regulating cellular function. Topo IIα shows cell-cycle specific expression during the S and G2/M phases and is essential for chromosome condensation. Topo IIα is closely related to the occurrence, development, diagnosis, prognosis and treatment of cancer. Topo IIβ is associated with non-proliferating function and believed to maintain the integrity of nuclear chromatin [4,5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
