Genistein, daidzein, and resveratrols stimulate PGC‐1β‐mediated gene expression

Abstract

PGC‐1β is a transcriptional co‐activator of nuclear receptors such as the estrogen receptor‐related receptor (ERR). Transgenic overexpression of PGC‐1β in mice increases energy expenditure and suppresses high‐fat diet‐induced obesity. In this study, we screened various food‐derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC‐1β. Soy‐derived isoflavones, genistein and daidzein, and several resveratrols activated PGC‐1β. Genistein, daidzein, and trans‐oxyresveratrol activated ERR‐responsive element‐mediated reporter activity in the presence of PGC‐1β. Stable overexpression of PGC‐1β in C2C12 myoblasts increased the expression of medium‐chain acyl‐CoA dehydrogenase (MCAD), an important enzyme in fatty acid β‐oxidation. Genistein and daidzein increased MCAD mRNA levels and mitochondrial content in PGC‐1β‐expressing C2C12 cells. These compounds activated ERR/PGC‐1β complex‐mediated gene expression, and our findings may be a practical foundation for developing functional foods targeting obesity.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.