Genistein adsorbed mesoporous bioactive glass with enhanced osteogenesis properties.

Abstract

Mesoporous bioactive glass (MBG) has good biocompatibility without immune reaction after implanting into tissue as biomaterial which was used in the treatment of bone defect. Genistein (G), a phytoestrogen, could be used in the treatment of osteoporosis in postmenopausal women. Here, we report that MBG with large pores (MBG-L) and MBG-L adsorbed with G (MBG-L/G) sustained-release G could enhance osteoblast differentiation and matrix mineralization. Interestingly, we observed that MBG-L enhanced the formation of bone-like deposit and Ca deposition in vitro. In the other side, we also found that MBG-L/G substrate could promote osteoblast differentiation and matrix mineralization through Erk activated Runx2 pathway. Interestingly, the expression of osteoblast-specific marker gene Osteopontin (Opn) was also increased in MC3T3-E1 cells cultured on MBG-L/G substrate. We conclude that MBG-L/G is a potential biomaterial for the treatment of bone defect.