Abstract
Eight Populus tremula and six P. tremuloides clones as well as 49 full-sib families were studied in GOT, LAP, 6-PGDH, and SKDH by horizontal starch gel electrophoresis. For GOT one polymorphic zone was found and segregation of seven full-sib families suggests Mendelian inheritance. For LAP and 6-PGDH two zones each were clearly scored. For LAP two polymorphic loci were proposed based on the phenotypic segregation of isozyme variants in six and 34 full-sib families, respectively. In 24 full-sibs families the presence of ‘null alleles’ was inferred for both loci. The genetic control of the upper zone of 6-PGDH was demonstrated by a segregation analysis of 17 full-sib families. SKDH also demonstrated a Mendelian inheritance pattern in 12 of the full-sib families analysed. The electrophoretic patterns of pollen were similar to those of buds, but migration rates of the supposed corresponding isozymes were slightly modified (Lap-B, Skdh, 6Pgdh-A). Lap-A was not present in pollen extracts and hybrid bands were not found when gels were stained for dimeric enzymes (6-PGDH, GOT).
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