Abstract
Genetic markers are needed for mating systems and breeding experiments in Cuphea lanceolata Ait.; however, none have been described in this species. Allozyme variation was analyzed among 14 F2 populations assayed for aconitase (ACO), diaphorase (DIA), esterase (EST), fluorescent esterase transaminase (FEST), glutamine oxaloacetate transaminase (GOT), menadione reductase (MNR), phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), and shikimate dehydrogenase (SKDH) enzyme activity. At least 23 loci were resolved in these enzyme systems: 6 monomorphic loci, 5 poorly resolved loci, and 12 clearly resolved polymorphic loci. Observed segregation ratios were generally not significantly different (P > 0.05) from expected segregation ratios; however, segregation distortion was observed at Skdh-1 and Mnr-1 (Dia-1) in some F2 populations. Skdh-1 and Pgm-2 and Est-1, Est-2, Fest-1, and Mnr-1 comprise putative linkage groups. Allozyme variation was observed between and within accessions. The expected average heterozygosity was 16.3%. There were one to eight polymorphic loci among the F2 populations analyzed. There were an average of 2.05 alleles per locus. Several useful codominant markers were identified and a partial allozyme linkage map was constructed. Additional work is needed to revise and complete the map.Key words: Cuphea, isozymes, goodness of fit test statistics, lauric acid, capric acid.
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