Abstract
Genetics is a powerful means of enhancing the beneficial traits of biological control agents. This is exemplified by the enhancement of tolerance of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora towards environmental extremes. The genetic approaches taken include selection (for heat tolerance and resistance to nematicides); screening for natural isolates displaying heat tolerance; hybridization (aided by genetic markers) and mutagenesis for generating mutants displaying either desired beneficial traits or marker mutations. Genetic engineering is a most promising tool for enhancing beneficial traits in biological control agents. The success in genetic transformation of EPN opens the way for generating transgenic nematodes carrying genes conferring resistance to various environmental extremes.
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