Genetics, cross-resistance and synergism of indoxacarb resistance in Helicoverpa armigera (Lepidoptera: Noctuidae).

Abstract

The cotton bollworm Helicoverpa armigera is a global pest of field and horticultural crops and has developed resistance to insecticides from many chemical classes. Indoxacarb is an important option for selective control of H. armigera in a range of crops that play host to this species. A strain of H. armigera resistant to indoxacarb (designated GY7-39) was detected from the field by F2 screening and characterised by comparison with a near-isogenic indoxacarb-susceptible laboratory strain to determine inheritance, cross-resistance profile and synergism of indoxacarb resistance. The level of indoxacarb resistance in the GY7-39 strain was 139-198-fold compared with the susceptible strain. Genetic analysis showed that resistance was autosomal, incompletely dominant and conferred by one or a few closely linked loci. Indoxacarb resistance in the GY7-39 strain did not confer cross-resistance to chlorantraniliprole. The GY7-39 strain was more susceptible to emamectin benzoate, fenvalerate, Cry1Ac and Cry2Ab compared with the susceptible strain. Indoxacarb resistance was synergised by the metabolic inhibitor PBO. Rapid selection of indoxacarb resistance in the GY7-39 strain indicates the potential risk of resistance development to indoxacarb in field populations of H. armigera. Lack of cross-resistance indicates that resistance could be managed effectively by the use of rotational strategies that incorporate transgenic technologies. Synergism studies indicate the potential involvement of metabolic detoxification enzymes as the mechanism of resistance to indoxacarb. © 2016 Society of Chemical Industry.