Abstract
Black rot caused by Xanthomonas campestris pv. campestris (Pam.) Dowson is the most destructive disease of cauliflower causing huge loss to the farmers throughout the world. Since there are limited sources of resistance to black rot in B. oleracea (C genome Brassica), exploration of A and B genomes of Brassica was planned as these were thought to be potential reservoirs of black rot resistance gene(s). In our search for new gene(s) for black rot resistance, F2 mapping population was developed in Brassica carinata (BBCC) by crossing NPC-17, a susceptible genotype with NPC-9, a resistant genotype. Out of 364 Intron length polymorphic markers and microsatellite primers used in this study, 41 distinguished the parental lines. However, resistant and susceptible bulks could be distinguished by three markers At1g70610, SSR Na14-G02 and At1g71865 which were used for genotyping of F2 mapping population. These markers were placed along the resistance gene, according to order, covering a distance of 36.30 cM. Intron length polymorphic markers At1g70610 and At1g71865 were found to be linked to black rot resistance locus (Xca1bc) at 6.2 and 12.8 cM distance, respectively. This is the first report of identification of markers linked to Xca1bc locus in Brassica carinata on B-7 linkage group. Intron length polymorphic markers provided a novel and attractive option for marker assisted selection due to high cross transferability and cost effectiveness for marker assisted alien gene introgression into cauliflower.
Highlights
Black rot caused by the gram negative bacterium Xanthomonas campestris pv. campestris (Xcc) (Pammel) Dowson is one of the most destructive diseases of vegetable Brassicas wherever this crop is grown [1,2]
The plants were scored at 30 DAI for disease reaction and grouped into resistant and susceptible categories based on disease severity and incidence (%)
The conclusion from the present study, is that resistance to black rot disease in B. carinata is governed by single dominant gene
Summary
Black rot caused by the gram negative bacterium Xanthomonas campestris pv. campestris (Xcc) (Pammel) Dowson is one of the most destructive diseases of vegetable Brassicas wherever this crop is grown [1,2]. Campestris (Xcc) (Pammel) Dowson is one of the most destructive diseases of vegetable Brassicas wherever this crop is grown [1,2]. This disease has a wide geographical distribution [3] causing severe. Molecular Mapping of Black Rot Resistance in Ethiopian Mustard damage to the cauliflower crop resulting in 10–50% yield loss under congenial environmental conditions [4]. It causes a systemic infection in susceptible plants as pathogen enters leaves through the hydathodes at leaf margins or injuries. Resistance breeding would be rewarding and environmentally safe approach by exploring available genetic sources harbouring useful resistance genes effective against this devastating pathogen
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