Abstract

India is one of the largest cotton-growing countries. Cotton is a fiber crop with varied applications from making tiny threads to fashionable clothing in the textile sector. In the near future, cotton crop will gain popularity as a multipurpose crop in India. The commercialization of Bt cotton in 2002 and consequently the fast adoption of Bt cotton hybrids by cotton farmers have enhanced the cotton production in India. Presently, genetically modified (GM) cotton has occupied 21.0 million hectares (mha) that comprise 14% of the global area under GM cultivation. In the coming years, improved cotton hybrids, with stacked and multiple gene events for improved fiber quality, insect resistance, drought tolerance, and herbicide tolerance, would further significantly improve the cotton production in India. With the dramatic increase in commercialization of GM crops, there is an urgent need to develop cost-effective and robust GM detection methods for effective risk assessment and management, post release monitoring, and to solve the legal disputes. DNA-based GM diagnostics are most robust assays due to their high sensitivity, specificity, and stability of DNA molecule.

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