Abstract
Previous work has shown that tissue cultures initiated from mature embryos of a specific experimental oat (Avena sativa L.) line, Gaf/Park, can be genetically engineered and fertile plants regenerated. The objectives of this study were to investigate the initiation of tissue cultures from mature embryos of 16 elite North American spring oat cultivars and to determine whether these tissue cultures can be used to produce transgenic plants. Fifteen of the genotypes tested produced at least some embryogenic callus that was similar in appearance to transformable callus. The elite cultivar Belle was tested to determine whether it could be genetically engineered. From 30 microprojectile bombardment treatments, 17 independently genetically engineered tissue cultures were produced that regenerated fertile, transgenic plants. These results indicate that the mature embryo‐derived tissue culture system will be useful for genetically engineering elite oat cultivars.
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