Abstract
Heavy metal removal using genetically engineered organisms (GEOs) offer more cost and energy-efficient, safer, greener, and environmentally-friendly opportunities as opposed to conventional strategies requiring hazardous or toxic chemicals, complex processes, and high pressure/temperature. Additionally, GEOs exhibited superior potentials for biosynthesis of nanoparticles with significant capabilities in bioreduction of heavy metal ions that get accumulated as nanocrystals of various shapes/dimensions. In this context, GEO-aided nanoparticle assembly and the related reaction conditions should be optimized. Such strategies encompassing biosynthesized nanoparticle conforming to the green chemistry precepts help minimize the deployment of toxic precursors and capitalize on the safety and sustainability of the ensuing nanoparticle. Different GEOs with improved uptake and appropriation of heavy metal ions potentials have been examined for bioreduction and biorecovery appliances, but effective implementation to industrial-scale practices is nearly absent. In this perspective, the recent developments in heavy metal removal and nanoparticle biosynthesis using GEOs are deliberated, focusing on important challenges and future directions.
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