Genetic variations in immunoglobulin G3 and association with staphylococcal intra-mammary infections in cattle and buffaloes

Abstract

Animals (n = 152) suffering with mastitis were used to study association between immunoglobulin G3 (IgG3) genotypes and staphylococcal mastitis. Thus, animals (affected and unaffected) were evaluated using PCR-RFLP. Restriction digestion of amplicons of IgG3 using BstYI showed allele A and, genotypes AC, AB and AA predominated in Karan Fries, Sahiwal and Murrah, respectively. HphI digestion revealed allele A and, genotypes AC and AB in higher frequency in animals of first group of all the breeds. Additionally, genotypes associated with mastitic infection showed predominance of AB (BstYI) in unaffected animals of Sahiwal and Murrah; whereas AC and AA were observed in affected group only. Genotype AB (HphI) was prevalent in unaffected and AC in affected animals of Karan Fries and Sahiwal. In Murrah, AC was common in affected and unaffected animal; while AB remained in affected category. Identified genotypes associated with determinants of SpA gene of S. aureus strains revealed the significant outcome. For example AB (BstYI) was found to be correalted with SpA ≤ 7R; whereas with SpA > 7R in Karan Fries. Genotypes AA and AB were more favorably associated with SpA ≤ 7R and AB with the SpA > 7R in Sahiwal cattle. The genotype AB seemed influenced (100%) with SpA > 7R and AC in SpA ≤ 7R in cases of Murrah. Similarly, AA (HphI) in Karan Fries was more likely to be correlated with SpA ≤ 7R, while AC with SpA > 7R. Overall, the molecular analysis revealed that IgG3 gene could be use for selection of animals against mastitis. However, further investigations on IgG3 needed to aid in identify disease- resistant animal.