Genetic variation of the Chinese longsnout catfish Leiocassis longirostris in the Yangtze River revealed using mitochondrial DNA cytochrome b sequences

Abstract

The mitochondrial DNA cytochrome b of 132 Leiocassis longirostris collected from 12 localities in the upper to lower reaches of the Yangtze River were amplified and partially sequenced using the PCR technique. The results showed that 27 nucleotide sites were variable along 817bp length of homologous sequence (3.3%), base substitutions happened mostly at the third codon position. A total of 22 haplotypes were identified, which were characterized with moderate haplotype diversity (h=0.5417±0.0519), but low nucleotide diversity (π=0.0019±0.0012). Median-joining network analysis revealed star-shaped patterns with one common central haplotype (H3), whereas mismatch distribution analysis found that the Chinese longsnout catfish fitted a smooth unimodal distribution, which suggested that this species underwent population expansion following bottlenecks and/or they originated from a small number of founding individuals. The time that the total population of Chinese longsnout catfish in the Yangtze River expanded was estimated 169,000–337,000years before present. The analysis of molecular variance (AMOVA) and phylogenetic reconstructions did not detect significant geographic structure between different river sections, especially between above and below the Gezhouba Dam and the Three Gorges Dam, which suggested that these recently developed dams might have not significantly resulted in population genetic differentiation in the Chinese longsnout catfish.