Abstract
In this paper, we adopted AFLP molecular marker technique to analyze the genetic differences of 85 individuals of Channa maculata , Channa algus and their hybrid ( C. maculata ♀× C. argus ♂), among which Channa maculata and Chonna argus was 30 respectively, their hybrid was 25. The results showed that tota1 of 459 bands were obtained by 11 primer combinations, of which 350 were polymorphic bands, the percentage of polymorphic loci was 76.25%, the average of polymorphic bands per primer combination was 31.8. There were 169 discrepant bands could be distinguished C. maculata and C. argus steadily, 78 of those were paternal ( C. argus ) specific bands and 72 were entailed on hybrid, 89 of those were maternal ( C. maculata ) specific bands and 71 were entailed on hybrid. In addition, there were 3 nonparental bands in hybrid. The results of analysis of molecular variance (AMOVA) found that the Nei’s unbiased measures of genetic identity between C. maculata and C. argus was 0.516 1, while those between hybrid and C. maculata , hybrid and C. argus was 0.718 9 and 0.747 6. And their Nei’s unbiased measures of genetic distance were respectively 0.661 5, 0.330 0 and 0.290 9, respectively, the analysis of molecular variance analysis (AMOVA) displayed that. There was significantly genetic differentiation among C. maculata , C. argus and the hybrid. On the other hand, the UPGMA cluster analysis revealed that, individuals of C. maculata and C. argus could be distinguished into two populations, but most individuals of hybrid scattered in populations of C. maculata and C. argus . In populations cluster analysis hybrid and C. argus populations were cluster firstly, then clustered with C. maculata . The preliminary analysis result indicated that the hybrid was supposed to be isolation cultivated because of high promiscuous possibility among C. maculata, C. argus and their hybrid. The results of this article would provide experimental evidence for genetic assaying of C. maculata , C. argus and their hybrid and the refference for reasonable utilization of their germplasms.
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