Abstract
Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines
Highlights
Gliadins are large families of wheat storage proteins with similar amino acid sequences
Nine allelic variants of gliadins were revealed among 62 Ukrainian bread wheat cultivars and 6 wheat lines created by M
DNA polymorphism of Gli-B1 locus examined in our research coincided with the diversity of allelic variants of gliadins, which were detected by acid polyacrylamide gel (APAGE) method in Ukrainian bread wheat cultivars, and divided all cultivars into six groups at the DNA level
Summary
Gliadins are large families of wheat storage proteins with similar amino acid sequences. Monomeric gliadin peptides are held together with glutenin polymers via intramolecular disulfide bonds and form gluten complex during mixing wheat grain flour and water [1]. Studying the gliadins gene sequences will advance the development of low gluten wheat by using RNA interference or Crispr/Cas 9 technologies in order to create safe food for people with this disease [4,5,6,7,8]. Gliadins are classified as α-, γ-, δ- and ω-gliadins, based on their electrophoretic mobility and primary amino acid sequence [9, 10]. The gliadin gene families are localized at six major loci on chromosomes of homoeologous group 1 and 6. Gli-1 loci are linked to LMW glutenin genes (Glu-3) and some important resistance genes, called R genes [11, 13, 15]
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