Genetic variation of COI gene of Hippa admirabilis in Northern Sulawesi

Abstract

Hippoid crab is crustacean species which inhabits sandy beach in tropical and subtropical area. One of them is Hippa admirabilis. Previously, this species was reported only in Sulawesi Island in Indonesia beside Taiwan and New Guinea. The aim of this study was to elucidate the genetic variation of Hippa admirabilis in Northern Sulawesi. Ten specimens were collected from two locations in the northern part of Sulawesi in 2016 which is Ogotumubu (Province of Central Sulawesi) and Gorontalo (Province of Gorontalo). The length of the amplified CO1 gene fragment is 596 bp. The obtained sequences are compared to our previous work in Genbank which is Hippa admirabilis from Banggai, Sulawesi. The phylogenetic tree was constructed to phylogeographic scenario based on Neighbor-Joining methods with Kimura 2-parameters models. The haplotype analysis was performed using DnaSP software. The phylogenetic tree shows that all of the H. admirabilis samples assembled into one clade. Five haplotypes of Hippa admirabilis was discovered in this study. There is one shared haplotype group with ten individuals from Gorontalo and Ogotumubu. The rest haplotype is exclusive belongs to each location. The nucleotide variation between Gorontalo, Ogotumubu, and Banggai (as reference sequence) was 8 nucleotide bases.