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Abstract
(...) Under the conditions employed in this study, restriction enzymes Bam HI, BclI, BstXI, and SmaI were particularly effective in yielding RFLP patterns that permit discrimination of strains. Restriction enzyme NheI was useful for differentiating pathogen strains that contain autonomously replicating pCS1 from those in which the plasmid is integrated into the bacterial chromosome. The RFLP patterns produced by NheI also provided evidence that integration of pCS1 into the chromosome is a site-specific process. No alterations in RFLP patterns were observed upon repeated subculture of selected strains. Numerical analysis of the RFLP data indicated that the strains are of high similarity
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