Abstract
Restriction analysis of the mitochondrial genome of 16 Japanese scallop (Patinopecten yessoensis) provided unique genotypes for each individual, mostly due to length variation. Fragment length variation was common, probably because tandemly repeated elements of noncoding mtDNA mutate to a different number of copies at high rates. To accurately estimate genetic diversity, we used the polymerase chain reaction (PCR) to selectively amplify coding mtDNA fragments and performed restriction analysis on these. Four pairs of PCR primers were used to amplify fragments encoding: (1) most of ATP synthetase subunit 6 and most of cytochrome c oxidase subunit 3, (2) part of cytochrome b apoenzyme, (3) tRNA for threonine, and (4) most of the large (16S) ribosomal gene. We used this technique to compare the genetic diversity of a population bred in a small experimental hatchery in British Columbia for three generations with its wild source population at Mutsu Bay, Aomori, Japan, and with a second wild population at Uchiura Bay, Hokkaido, Japan. The three populations were similar in the frequency distributions of the 11 mitochondrial clonal lines. This suggests that the experimental hatchery stock was not severely inbred and that gene flow between the two wild Japanese populations has been sufficient to prevent divergence.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Canadian Journal of Fisheries and Aquatic Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.