Abstract
Prosthetic Joint Infection (PJI) is a serious complication of Total Joint Arthroplasty (TJA). The Th-17 immune response characterised by IL (interleukin)-17A, IL-17F, IL-23, chemotactic cytokines and their receptors, plays a prominent role in the immune response to invading bacteria. In addition, high expression of IL-17A has been reported in PJI. The aim of this study was to investigate whether genetic variation in the key molecules of the Th-17 immune response can affect the risk for PJI. Altogether ten Single Nucleotide Polymorphisms (SNPs) of IL17A (rs2275913), IL17F (rs763780), IL4 (rs2243250), IL12A (rs583911), IL12B (rs3212227 and (rs17860508), IL23R (rs7517847), CXCL1 (rs4074), CXCL5 (rs425535) and CXCR2 (rs2230054) genes were genotyped by PCR with sequence specific primers (SSP) in 98 patients with PJI and two control groups 1) an aseptic TJA control (253 patients with TJA that did not develop PJI at least 6 yrs. after the surgery) and 2) a population control (185 healthy control subjects without TJA). Allele, genotype and phenotype frequencies of investigated SNPs did not differ between the patients with PJI and control patients with aseptic TJA (p>0.05). There was no difference in the distribution of tested SNPs between patients with PJI and population controls without TJA (p>0.05) or between the two controls groups (p>0.05). We cannot nominate any of studied polymorphisms in IL17A, IL17F, IL4, IL12A, IL12B, IL23R, CXCL1, CXCL5 and CXCR2 genes as risk factors for PJI in the Czech TJA patients examined.
Highlights
Prosthetic Joint Infection (PJI) is a serious complication after Total Joint Arthroplasty (TJA) that usually leads to clinical failure requiring surgical revision and long-term antibiotic therapy
We investigated whether selected polymorphisms of the genes associated with Th17 signalling, namely IL17A, IL17F, IL4, IL12A, IL12B, IL23R, CXCL1, CXCL5 and CXCR2, could be associated with the risk of PJI
Genotype and phenotype frequencies of ten investigated polymorphisms located within nine genes encoding for IL-17A, IL-17F, IL-4, IL-12A, IL-12B, IL-23R, CXCL1, CXCL5 and CXCR2 molecules did not differ between the patients with PJI and control patients with aseptic TJA (P>0.05 for all Single Nucleotide Polymorphisms (SNPs); Table 3)
Summary
Prosthetic Joint Infection (PJI) is a serious complication after Total Joint Arthroplasty (TJA) that usually leads to clinical failure requiring surgical revision and long-term antibiotic therapy. Expression and function of the cytokines and chemokines related to Th-17 immune response are known to be affected by genetic factors such as Single Nucleotide Polymorphisms (SNPs) (ref.[10]). Ten Single Nucleotide Polymorphisms (SNPs) of IL17A (rs2275913), IL17F (rs763780), IL4 (rs2243250), IL12A (rs583911), IL12B (rs3212227 and (rs17860508), IL23R (rs7517847), CXCL1 (rs4074), CXCL5 (rs425535) and CXCR2 (rs2230054) genes were genotyped by PCR with sequence specific primers (SSP) in 98 patients with PJI and two control groups 1) an aseptic TJA control We cannot nominate any of studied polymorphisms in IL17A, IL17F, IL4, IL12A, IL12B, IL23R, CXCL1, CXCL5 and CXCR2 genes as risk factors for PJI in the Czech TJA patients examined
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