Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

Agus Nuryanto,Kusbiyanto Kusbiyanto,Petrus Hary Tjahja Soedibja,Hendro Pramono,Nael Huda Qonita

doi:10.15294/biosaintifika.v9i2.9301

Agus Nuryanto, Kusbiyanto Kusbiyanto + Show 3 more

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https://doi.org/10.15294/biosaintifika.v9i2.9301

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Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. Ther result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315bp, and 210bp, and also Cytochrome b-AluI of 334bp and 189bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366bp, and 159bp and Cytochrome b-AluI 525bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.

Highlights

Giant gourami (Osphronemus goramy Lacepede, 1801) has been widely cultivated in Indonesia for many years
This study aims to develop PCR-RFLP marker to be used as specific marker for large and small individulas differentiation within population of soang gourami fingerling from single spawning
Restriction fragments are wellknown as PCR-RFLP markers

Summary

Introduction

Giant gourami (Osphronemus goramy Lacepede, 1801) has been widely cultivated in Indonesia for many years. This species has high economic value due to its taste meat (Azrita, 2015). If we examined carefully in the fingerling of soang strain from single spawning, their body sizes are varies. These body size variations are suggested because of each individual have different growth rates. According to Kartikaningsih et al (2001), genetic factors are among various factors that cause giant gourami growth rate is relatively slower than other fish species. Previous study proved that variation on growth hormone gene was possitivelly corelated with individual body size in certain population (Hua et al, 2009)

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