Genetic variation in body weight gain and composition in the intercross of Large (LG/J) and Small (SM/J) inbred strains of mice

Abstract

Strain intercross experiments provide a powerful means for mapping genes affecting complex quantitative traits. We report on the genetic variability of the intercross of the Large (LG/J) and Small (SM/J) inbred mouse strains as a guide to gene mapping studies. Ten SM/J males were crossed to 10 LG/J females, after which animals were randomly mated to produce F1, F2, and F3 intercross generations. The 1632 F3 animals from 200 full-sib families were used to estimate heritabilities and genetic correlations of the traits measured. A subset of families was cross-fostered at birth to allow measurement of the importance of post-natal maternal effects. Data was collected on weekly body weight from one to 10 weeks and on organ weights, body weight, reproductive fat pad weight, and tail length at necropsy in the intercross generations. There was no heterosis for age-specific weights or necropsy traits, except that one-week weight was the highest in the F2 generation, indicating heterosis for maternal effect in the F1 mothers. We found moderate to high heritability for most age-specific weights and necropsy traits. Maternal effects were significant for age-specific weights from one to four weeks but disappeared completely at ten-week weight. Maternal effects for necropsy traits were low and not statistically significant. Age-specific weights showed a typical correlation pattern, with correlation declining as the difference in ages increased. Among necropsy traits, reproductive fat pad and body weights were very highly genetically correlated. Most other genetic correlations were low to moderate. The intercross between SM/J and LG/J inbred mouse strains provides a valuable resource for mapping quantitative trait loci for body size, composition, and morphology