Genetic variation for flowering time and height reducing genes and important traits in western Canadian spring wheat

Abstract

Genetic variation is prerequisite for wheat improvement. High grain yield and protein content and early maturity are some of the major objectives in global as well as Canadian wheat breeding programs. We investigated genetic diversity for earliness related and plant height reducing (Rht) genes in 82 spring wheat cultivars, registered in western Canada, through eight diagnostic DNA markers. Allelic variation was observed at the Vrn-A1, Vrn-B1, Vrn-D1 and Ppd-D1 loci but not at Ppd-A1 and Ppd-B1 loci in the 82 cultivars. Spring type allele of Vrn-A1 was present in 94 % cultivars, whereas only two cultivars carried spring allele of Vrn-D1. Among the four earliness related genes, the most frequent combination was Vrn-A1a, Vrn-B1, vrn-D1 and Ppd-D1b, which was found in 32 cultivars. As for the Rht genes, eight cultivars had Rht-B1b and 13 cultivars had Rht-D1b. All cultivars carrying dominant allele of Vrn-B1, photoperiod-insensitive allele of Ppd-D1 and height reducing allele of Rht-1 had shorter plants and higher grain yield but lower grain protein content. Days to heading and maturity showed positive genetic (rg = 0.65) and phenotypic (rp = 0.44) correlation, and were also positively correlated with grain yield and kernel weight but negatively correlated with test weight and protein content. Plant height was positively correlated with grain protein content (rg = 0.53; rp = 0.42), but negatively correlated with grain yield (rg = −0.47; rp = −0.14). Grain yield and protein content showed negative genetic correlation (rg = −0.57). Among the sixty cultivars from Canada Western Red Spring Class released over 100 years, the newest cultivar yielded 23 % more grain and had 15 % higher grain protein than the oldest cultivar ‘Red Fife’. Breeders in western Canada have incorporated vernalization and photoperiod insensitive and Rht genes in modern cultivars to promote early maturity, to make use of off-season nurseries abroad, and to improve lodging tolerance.