Abstract

Collection of the parasitoid Microctonus hyperodae from diverse habitats in South America was undertaken to maximise the genetic diversity of parasitoids released in New Zealand. Laboratory studies have shown that the populations varied significantly in such aspects as fecundity, photoperiodic response and morphometrics. The use of tandem repeat primers and PCR to amplify anonymous DNA regions has now enabled differentiation of the populations into two genetically distinct types due to the presence or absence of an as yet unidentified DNA region of approximately 480bp. Using this method, parasitoids can be classified as originating from either the east or west of the Andes. This provides the first molecular evidence of intraspecific genetic variation within M. hyperodae.

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