Abstract
The assessment of genetic diversity among the members of a species is of vital importance for successful breeding and adaptability. In the present study 40 old genotypes of maize from Hungary, Union of Soviet Socialist Republics, Poland, Czechoslovakia, Yugoslavia and Slovak Republic were evaluated for the total seed storage proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) through vertical slab unit. The number of total scorable protein bands was twentythree as a result of SDS-PAGE technique but those that were not cosistent in reproducibility and showed occasional variation in sharpness and density were not considered. Out of twentythree polypeptide bands, 6 (31%) were commonly present in all accessions and considered as monomorphic, while 17 (65%) showed variations and considered as polymorphic. On the basis of banding profiles of proteins of different kDa, gel was divided into zones A, B and C. The major protein bands were lied in zones A and B, while minor bands were present in zones C. In zone A out of 10 protein bands, 1 were monomorphic and 9 were polymorphic. In zone B out of 8 protein bands, 3 was monomorphic and 5 was polymorphic and in zone C out of 5 protein bands, 2 were monomorphic whereas 3 polymorphic. The dendrogram tree demonstrated the relationship among the forty registered old maize genotypes according to the similarity index, using UPGMA cluster analysis. The dendrogram was divided into two main clusters. The first one contained eleven genotypes from maize, while the second cluster contained the twentynine genotypes of maize. Similarly the present study of genetic variability in the seed storage polypeptide determined by SDS-PAGE technique proved that it is fruitful to identify genetic diversity among accessions of maize.
Highlights
Maize (Zea mays L.) is an annual, cross-pollinated by wind and the only monoecious among cereal crops to have male and female inflorescences on separate branches of the same plant
The objectives were to find out the level of genetic variability present in 40 maize germplasm by using the electrophoretic profiles of total seed proteins with different molecular weights through sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE)
The dendrogram was divided into two main clusters
Summary
Maize (Zea mays L.) is an annual, cross-pollinated by wind and the only monoecious among cereal crops to have male and female inflorescences on separate branches of the same plant. It belongs to grass family Poaceae (Gramineae) which is leading in importance in the order Poales (Bremer et al, 2003). Maize seed consists of two types of protein i.e., zein and non-zein protein. The objectives were to find out the level of genetic variability present in 40 maize germplasm by using the electrophoretic profiles of total seed proteins with different molecular weights through SDS-PAGE. Gel was scanned with densitometer GS 800 (Bio-Rad) and evaluated with Quantity One-1D Analysis Software
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