GENETIC VARIATION AND RELATEDNESS AMONG HIGH YIELDING RICE VARIETIES (Oryza sativa L.) REVEALED BY RAPD MARKERS

F Easmin,M A Samad,M S Alam,M S Islam,M S Rahman

doi:10.3329/bjpbg.v21i1.17042

F Easmin, M A Samad + Show 3 more

Open Access

https://doi.org/10.3329/bjpbg.v21i1.17042

Copy DOI

Abstract

Genetic variation is a principal concern for the plant breeders. Genetic variation and relationship among high yielding rice varieties viz. Binadhan 4, Binadhan 5, Binadhan 6, Binasail, BRRI dhan28 and BRRI dhan29 were analyzed using four decamer random primers. Polymerase Chain Reaction (PCR) amplified 22 RAPD markers, of which 18 (81.82%) were polymorphic. The proportion of polymorphic loci and the gene diversity values were 59.09% and 0.25 for the Binadhan 4; 59.09% and 0.21 for Binadhan 6; 54.55% and 0.23 for Binasail; 54.55% and 0.19 for BRRI dhan29; 50.00% and 0.19 for Binadhan 5 and 45.45% and 0.18 for BRRI dhan28, respectively. The coefficient of gene differentiation (Gst) across all loci was calculated as 0.35 reflecting the existence of high level of genetic variation among the six modern rice varieties. UPGMA dendrogram based on Neis genetic distance segregated the six high yielding rice varieties into two clusters: all four mutant varieties viz. Binadhan 4, Binadhan 5, Binadhan 6 and Binasail formed one cluster and two varieties of BRRI grown in boro season, BRRI dhan28 and BRRI dhan29 grouped together in another cluster. Among the mutants, two boro season varieties, developed from the same parent, Binadhan 5 and Binadhan 6 grouped together with genetic distance of 0.10. Therefore, RAPD offer a reliable method to evaluate genetic variation and relatedness among the high yielding rice varieties.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17042

Highlights

Rice (Oryza sativa L.) is the most important and extensively grown food crop leading cereal in the world including Bangladesh
Six high yielding rice varieties viz. Binadhan 4, Binadhan 5, Binadhan 6, Binasail, Bangladesh Rice Research Institute (BRRI) dhan28 and BRRI dhan29 were used in the present study
The seeds of the varieties were collected from Genetic Resource Centre (GRS), Bangladesh Rice Research Institute (BRRI) and Bangladesh Institute of Nuclear Agriculture (BINA)

Summary

Introduction

Rice (Oryza sativa L.) is the most important and extensively grown food crop leading cereal in the world including Bangladesh. Over the last two decades the entire growth in rice production has been increased mainly due to the conversion of local to high yielding varieties (HYVs) (World Bank, 1995). HYV rice is developed from local varieties through different improved breeding programs. Intra- or inter-specific genetic variation among different varieties plays a key role in the improvement of grain through the breeding programs. Knowledge of genetic variation is essential in any breeding program. Plant genetic diversity is a key component of any agricultural production system. This genetic diversity or similarity is measured through genetic markers and is used to determine evolutionary relationship within and between species, genera or higher taxonomic

Objectives

Methods

Results