Abstract
Key messageIdentification of QTL for phytosterol content, oil content, fatty acids content, protein content of defatted meal, and seed weight by multiple interval mapping in aBrassica napusDH population.Phytosterols are minor seed constituents in oilseed rape which have recently drawn wide-interest from the food and nutrition industry due to their health benefit in lowering LDL cholesterol in humans. To understand the genetic basis of phytosterol content and its relationship with other seed quality traits in oilseed rape, QTL mapping was performed in a segregating DH population derived from the cross of two winter oilseed rape varieties, Sansibar and Oase, termed SODH population. Both parental lines are of canola quality which differ in phytosterol and oil content in seed. A genetic map was constructed for SODH population based on a total of 1638 markers organized in 23 linkage groups and covering a map length of 2350 cM with a mean marker interval of 2.0 cM. The SODH population and the parental lines were cultivated at six environments in Europe and were phenotyped for phytosterol content, oil content, fatty acids content, protein content of the defatted meal, and seed weight. Multiple interval mapping identified between one and six QTL for nine phytosterol traits, between two and six QTL for four fatty acids, five QTL for oil content, four QTL for protein content of defatted meal, and three QTL for seed weight. Colocalizations of QTL for different traits were more frequently observed than individual isolated QTL. Major QTL (R2 ≥ 25 %) were all located in the A genome, and the possible candidate genes were investigated by physical localization of the QTL to the reference genome sequence of Brassica rapa.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-015-2621-y) contains supplementary material, which is available to authorized users.
Highlights
Oilseed rape (Brassica napus L.; genome AACC, 2n = 38) is the world’s third-leading source of vegetable oil for human nutrition and industrial products
Polymorphism of molecular markers and linkage map development of SODH population Different types of molecular markers were used in the construction of the genetic map for the SODH population: amplified fragment length polymorphism (AFLP), Simple sequence repeats (SSR), Diversity arrays technology (DArT), Silicor-DArT, Single nucleotide polymorphism (SNP), Kompetitive allele specific PCR (KASP), and candidate gene-based markers
With 16 AFLP primer combinations, a total of 75 polymorphic markers could be scored in the SODH population
Summary
In the case of a complex trait like seed oil content, the number of QTL as reported by numerous studies varied between 3 and 27 QTL and were found distributed among 17 of the 19 chromosomes in B. napus (Rahman et al 2013). These QTL individually explained between 2 and 10 % of the phenotypic variance while the additive effect ranged from 0.2 % to more than 1.0 % (Rahman et al 2013). The canola quality oilseed rape possesses a nearly ideal fatty acid profile, there is still room for improvement on the thermal stability of oil by further increasing oleic acid and reducing the polyunsaturated fatty acids content
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