Abstract
Maize is a very fertile and widely environmental grown crop and it globally cultivated. The purpose of our research was to determine genetic variation among three Zea mays L. cultivars (Al maha, Drachma and Talar F-1). The primer ITS1 and ITS4 used as a molecular marker in a conventional Polymerase Chain Reaction (PCR) with a 290 bp ampli- fication outcome. The nucleotide sequences of amplification products were analyzed, sequence alignment was significantly revealed which confirming Zea mays diagnosis. Furthermore, analysis of genetic relationship revealed a neighboring relationship between Talar F-1 and Almaha cultivars(93),whereas phylogeny schematic diagram clearly showed presence of Drachma cultivar in other cluster(77).
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