Abstract
Genetic diversity among and within 10 populations of Iranian alfalfa, from different areas of Azarbaijan, Iran was analyzed by screening DNA from seeds of individual plants and bulk samples. In individual study, 10 randomly amplified polymorphic DNA (RAPD) primers produced 156 polymorphic bands and a high level of genetic diversity was observed within populations. The averages of total and within population genetic diversity were 0.2349 and 0.1892, respectively. Results of analysis of molecular variance (AMOVA) showed the great genetic variation existed within populations (81.37%). These Results were in agreement with allogamous and polyploid nature of alfalfa. Cluster analysis was performed based on Nei’s genetic distances resulting in grouping into 3 clusters which could separate breeding population from other populations. Results of cluster analysis were in consistent with morphological and geographical patterns of populations. The results of bulk method were different from individual analysis. Our results showed that RAPD analysis is a suitable method to study genetic diversity and relationships among alfalfa populations.Keywords: Alfalfa; RAPD; Genetic diversity; Analysis of Molecular Variance; Cluster analysis.DOI: http://dx.doi.org/10.3329/bjpt.v18i2.9296Bangladesh J. Plant Taxon. 18: (2): 93-104, 2011 (December)
Highlights
Alfalfa (Medicago sativa L.) is the most important forage legume (Veronesi et al, 2010), originated in Caucasus, northeastern Turkey, northwestern Iran and Turkmenistan (DehghanShoar et al, 1997), though Iran is known as central origin (Hanson, 1988)
Alfalfa cultivars are synthetic varieties developed by intercrossing the selected parents and advancing their offspring through three or four generations of seed increase (Rowe and Hill, 1999). Genetic studies such as differentiation between cultivars and estimating the genetic diversity within and between populations are important in alfalfa breeding programs to use some of these populations as selected parents and producing higher yielding cultivars (Veronesi et al, 2010)
Since reproducibility is an important factor in randomly amplified polymorphic DNA (RAPD) studies (Ulloa et al, 2003), only reproducible bands were used in present investigation
Summary
Alfalfa (Medicago sativa L.) is the most important forage legume (Veronesi et al, 2010), originated in Caucasus, northeastern Turkey, northwestern Iran and Turkmenistan (DehghanShoar et al, 1997), though Iran is known as central origin (Hanson, 1988) It is an autotetraploid and allogamous plant (Flajoulot et al, 2005). Alfalfa cultivars are synthetic varieties developed by intercrossing the selected parents and advancing their offspring through three or four generations of seed increase (Rowe and Hill, 1999) Genetic studies such as differentiation between cultivars and estimating the genetic diversity within and between populations are important in alfalfa breeding programs to use some of these populations as selected parents and producing higher yielding cultivars (Veronesi et al, 2010)
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