Genetic variation among asexual progeny of Phytophthora infestans detected with RAPD and AFLP markers

Abstract

Genotypic variation among 32 single‐zoospore isolates (SZI) of Phytophthora infestans, derived asexually from two hyphal‐tip parental isolates (PI‐105 and PI‐1) of the US‐8 genotype, was assessed with 80 random amplified polymorphic DNA (RAPD) primers and 18 amplified fragment length polymorphic DNA (AFLP) primer pairs. In previous investigations, the SZIs from parental isolate PI‐105 showed high levels of virulence variability and were differentiated into 14 races, whereas the SZIs from PI‐1 showed identical virulence to the parent. The purpose of this investigation was to determine if phenotypic variation observed among SZIs of P. infestans could be detected at the DNA level in these isolates. Polymorphism was detected with 51 RAPD primers and with all 18 AFLP primer pairs in PI‐105 SZIs. In SZIs from PI‐1, polymorphism was also detected with 25 RAPD primers and 17 AFLP primer pairs. Cluster analysis using the unweighted pair‐group method with arithmetic averages (UPGMA) separated the SZIs from parent PI‐105 into six virulence groups, 11 RAPD groups and three AFLP groups. Cluster analysis of PI‐1 SZIs, which all belong to the same virulence group, differentiated them into four RAPD groups and six AFLP groups. No close correlation among RAPD, AFLP and virulence groups could be established within the two progenies of SZIs. Results of this study suggest that there is a considerable level of inherent genetic variability among SZIs derived asexually from the same parental isolate. The possible mechanisms and implications of this genetic variation are discussed.