Abstract
Non-syndromic tooth agenesis (or non-syndromic congenitally missing tooth) is one of the most common congenital defects in humans affecting the craniofacial function and appearance. Single nucleotide polymorphisms (SNPs) have been associated with an individual’s susceptibility to these anomalies. The aim of the present study was therefore to investigate the roles of the potentially functional SNPs of BMP2 in the occurrence of tooth agenesis. Overall, four potentially functional SNPs of BMP2 (rs15705, rs235768, rs235769 and rs3178250) were selected, and their associations with the susceptibility of tooth agenesis were evaluated in a case-control study of 335 non-syndromic tooth agenesis cases and 444 healthy controls. The SNPs rs15705 and rs3178250 were found to be associated with an individual’s risk of tooth agenesis (P = 0.046 and P = 0.039, respectively). Both SNPs showed an increased risk of mandibular incisor agenesis (rs15705, AA/AC vs. CC = 1.58, 95% CI = [1.06–2.34], P = 0.024; rs3178250, TT/TC vs. CC = 1.60, 95% CI = [1.08–2.37], P = 0.020). Bioinformatics analysis indicated that these two SNPs located at the 3’-untranslated region (3’-UTR) of BMP2 might alter the binding ability of miR-1273d and miR-4639-5p, respectively, which was confirmed by luciferase activity assays in the 293A and COS7 cell lines (P < 0.001 in 293A and P < 0.01 in COS7 for miR-1273d; and P < 0.001 in both cells for miR-4639-5p). Furthermore, BMP2 mRNA expression decreased after transfecting either miR-1273d or miR-4639-5p into these two cell lines (P < 0.01 in 293A and P < 0.001 in COS7 for miR-1273d, and P < 0.01 in both cell lines for miR-4639-5p). Taken together, our findings indicate that rs15705 and rs317250 are associated with the susceptibility of non-syndromic tooth agenesis by possibly affecting miRNAs and mRNA interaction.
Highlights
Tooth agenesis is one of the most common congenital defects in humans, and it may affect individual’s appearance, chewing ability, speech, facial development and overall health
Single nucleotide polymorphisms (SNPs) rs15705, rs235769 and rs3178250 were located in the 3’-untranslated region (3’-UTR), while SNP rs235768 was located in the exon of BMP2
Based on the single SNP analysis, we found that rs15705 and rs3178250 were potentially associated with an increased susceptibility to tooth agenesis, and we conducted further subgroup analysis according to the position and severity of tooth agenesis
Summary
Tooth agenesis (or congenitally missing tooth) is one of the most common congenital defects in humans, and it may affect individual’s appearance, chewing ability, speech, facial development and overall health. For Caucasians, the most common congenitally missing teeth (excluding the third molars) are the second mandibular premolars, the maxillary lateral incisors, and the maxillary second premolars [3]. Syndromic tooth agenesis refers to complex developing syndromes associated with a congenitally missing tooth or teeth, such as non-lethal Raine syndrome [4], cleft lip and palate [5] and HATS syndrome [6]. Non-syndromic tooth agenesis typically involves a congenitally missing tooth in an isolated form without any other major birth defects
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