Genetic variability of the storage protein loci in the european winter common wheat varieties licensed for growing in Ukraine

Abstract

Aim. The aim of the study was to investigate genetic variability of storage protein loci in European common winter wheat varieties licensed for growing in Ukraine in 2018 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the high-molecular-weight (HMW) glutenin loci Glu-1 and gliadin loci Gli-1. Results. Genotypes at the gliadin loci Gli-A1, Gli-B1, Gli-D1 and HMW glutenin loci Glu-A1, Glu-B1, Glu-D1 were identified in 48 winter common wheat varieties. A total of twenty gliadin alleles (including 10 at the Gli-A1, 4 at the Gli-B1, 6 at the Gli-D1 loci) and eleven HMW-GS alleles (including 3 at the Glu-A1, 5 at the Glu-B1, 3 at the Glu-D1 loci) were revealed. Differences in frequencies of alleles at storage protein loci were established. Conclusions. The high level of allelic variation was observed at the Gli-1 and Glu-1 loci in European common winter wheat varieties. Predominant alleles were revealed: Gli-A1о, Gli-A1а, Gli-A1f, Gli-B1f, Gli-D1b, Gli-D1d, Glu-А1c, Glu-А1a, Glu-B1с, Glu-B1d, Glu-D1d, Glu-D1a. Differences in frequencies of alleles at the storage protein loci between European and Ukrainian common winter wheat varieties were found. Keywords: Triticum aestivum L., winter wheat, storage protein, alleles.