Abstract
A total of 240 patients with chronic hepatitis B virus were included in the study, including 124 men and 116 females ranging in age from 6 to 78 years, PCR was used to amplify the Hepatitis B viral gene segment. There were only 26 positive samples for PCR amplification of a gene HBV S with strong positive bands submitted for sequencing. The NCBI BLASTn engine showed about 98% to 99% sequences similarities between the sequenced samples and the intended reference target sequences. By comparing the observed nucleic acid sequences of these investigated samples with the retrieved nucleic acid sequences (GenBank acc. MK840532.1), the details of its sequences were highlighted, and the total length of the amplified amplicons was also determined, the alignment results of the 548 bp samples revealed the presence of ten nucleic acid variations represented by ten nucleic acid substitutions in the analyzed samples in comparison with the most similar referring reference nucleic acid sequences (GenBank acc. no. MK840532.1), Our results indicated the presence of ten nucleic acid variants observed in the investigated samples, namely 163C>T, 184T>C, 210T>C, 217C>A, 274A>G, 310T>C, 336G>A, 346G>A, 372A>T, and 405A>C.
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