Abstract
Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to dev elop effective methods of control, it is necessary to do cument its genetic diversity. Approach: The objective of this study was to analyze the genetic variabilit y of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism (AFLP). Results: In the selective amplification using eight primer combinations we obtained a total of 446 AFLP markers with a 100% polymorphism. Out of 41 isolates, 36 different AFLP patterns were observed and five were replicates of the same pattern. The dendrogram shows inter- and intrapopulation similarity indexes of 0.26, 0.98 an d 0.31, 0.98, respectively. Six groups emerged from the principal components analysis, five of which were c learly defined, while the other one was spread out. Conclusion: We conclude that R. solani growing in Sonoran vineyards shows a high degree of genetic variability, even under similar environmental condi tions.
Highlights
Frank (Donk), its taxonomic classification is still unresolved (Gonzalez-Hernandez, 2002). This is the reason that the hyphal anastomosis reaction has been used to group relatively homogeneous groups; the fusion of hyphae indicates that both belong to the same anastomosis group
The variability in the degree of hyphal fusion, morphology, pathogenicity and host range observed in AGs (AG-1, AG-13) has made it necessary to define intraspecific groups, reducing the usefulness of hyphal anastomosis for R. solani classification
The lack of congruence observed between anastomosis and vegetative compatibility could indicate that the points evaluated correspond to a C2 reaction and the strains compared belong to the same anastomosis group but to a different vegetative compatibility group
Summary
The aim of this study was to analyze the genetic was removed and placed on a slide to determine the variability of R. solani isolated from the rhizosphere of number and type of compatibility reactions Mycelial growth was observed with a light microscope hyphal tips were Extraction of genomic DNA: Dry mycelium was used selected from individuals with characteristics similar to to extract DNA as reported by Raeder and Broda (1985) Those of Rhizoctonia and inoculated on nutritious with slightly modifications on the extraction buffer that medium (yeast-glucose extract) to develop pure strains. The labeled-amplified isolates from La Costa de Hermosillo, while the isolates fragments were automatically captured and recorded from Pesqueira are distributed among the remaining during electrophoresis using an automatic IR2 groups and did not show a specific pattern with respect sequencer (LiCor, Lincoln, Nebraska).
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